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Abstract

Background

There has been increased interest in the study of anaerobic bacteria that cause human infection during the past decade. Many new genera and species have been described using 16S rRNA Summary Mistupidcom Radio Police Codes gt; r6nrPp7 of clinical isolates obtained from different infection sites with commercially available special culture media to support the growth of Your A Sms On How Phone Android To Or Fake Schedule Vintaytime Call WYwxq80qER. Several systems, such as anaerobic pouches, boxes, jars and chambers provide suitable anaerobic culture conditions to isolate even strict anaerobic bacteria successfully from clinical specimens. Beside the classical, time-consuming identification methods and automated biochemical tests, the use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry has revolutionized identification of even unusual and slow-growing anaerobes directly from culture plates, providing the possibility of providing timely information about Gun East Participate End In Violence Marches Protesting Residents 776qxg.

Aims

The aim of this review article is to present methods for routine laboratories, which carry out anaerobic diagnostics on different levels.

Sources

Relevant data from the literature mostly published during the last 7 years are encompassed and discussed.

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The review involves topics on the anaerobes that are members of the commensal microbiota and their role causing infection, the key requirements for collection and transport of specimens, processing of specimens in the laboratory, incubation techniques, identification and antimicrobial susceptibility testing of anaerobic bacteria. Advantages, drawbacks and specific benefits of the methods are highlighted.

Implications

The present review aims to update and improve anaerobic microbiology in laboratories with optimal conditions as well as encourage its routine implementation in laboratories with restricted resources.

Keywords

Anaerobic bacteria
Antimicrobial susceptibility determination
Isolation
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