The aim of this review article is to present methods for routine laboratories, which carry out anaerobic diagnostics on different levels.
Relevant data from the literature mostly published during the last 7 years are encompassed and discussed.
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The review involves topics on the anaerobes that are members of the commensalmicrobiota and their role causing infection, the key requirements for collection and transport of specimens, processing of specimens in the laboratory, incubation techniques, identification and antimicrobial susceptibility testing of anaerobic bacteria. Advantages, drawbacks and specific benefits of the methods are highlighted.
The present review aims to update and improve anaerobic microbiology in laboratories with optimal conditions as well as encourage its routine implementation in laboratories with restricted resources.